Production of Podophyllotoxin An Anti-Cancer Drug, By Plant Cell Fermentation
Introduction
Anti cancer drug podophyllotoxin is currently extracted from the roots of the whole plant Podophyllum hexandrum (an endangered species) which could lead to its extinction in due course of time. Further the plant grows only in the Himalayan region. Mass propagation of the plant tissue culture could provide a solution. to the problem and also the much needed drug. The root explants of the germinated seedlings of P.hexandrum were used successfully to develop the callus as well as the suspension culture of the cells. The effect of the culture and environmental parameters has been studied to produce the drug at high conc. in the suspension culture. The application of fed batch culture strategy has further enhanced the productivity of the drug in lab - scale bioreactor.
Special Features
The drug can be produced by fermentation process using plant cells instead of traditional way of cutting the whole plant and extracting the drug from it.
Prospective Users
Drug company
Keywords
Podophyllotoxin, Podophyllum hexandrum, plant cell fermentation
Glossary
Podophyllum hexandrum - A plant species belonging to Berberidanceae family. It grows at an altitude of about 4000 meters in the Himalayan range. In the naturalplant, it synthesizes the anti-cancer drug, podophyllotoxin, in its roots; Fermentation- A process which employs biological agents (microorganisms, plants, animals or enzymes) for production of primary or secondary metabolites (alcohols, organic acids, antibiotics, enzymes, pharmaceuticals, alkaloids, flavours etc.) in a fermenter/bioreactor under optimal culture conditions.
Developed by : Dr. K A Srivastava
Department: Biochemical Engineering and Biotechnology
For further information please contact
Managing Director
Foundation for Innovation and Technology Transfer (FITT)
Indian Institute of Technology, Delhi
Hauz Khas, New Delhi-110016, INDIA
Tel : 91-011-26597167, 26857762, 26581013, 26597153
Fax : 91-011-26851169
E-mail : drkdpn@gmail.com
