Transgenic Sugarcane Model for Production of High Value Biomolecules
Introduction
The technology offers a single-pot two-step process for synthesis of highly functionalized piperidines in stereochemically pure forms. Synthesis of 2,6- disubstituted piperidines in diastereo as well as eThe technology offers a transgenic sugarcane model for production high value protein molecules eg: pharmaceutical proteins like vaccines, therapeutic proteins, oral vaccines, or any other intermediate proteins of industrial value. The transgenic sugarcane model has been developed at Sugarcane Breeding Institute, Coimbatore, India.
Business Opportunity
BCIL is looking for industrial partners keen to license the transgenic sugarcane model or validate the transgenic model for production of high value protein through option agreement.
State of Development
The transgenic sugarcane model has been successfully used for the production of Betaglucuronidase (gus) protein. The yield of partially purified GUS protein is 1mg/ml of juice. The estimated purity is 70%.
Competitive Advantages
The transgenic sugarcane model was used for production of Beta-glucuronidase (gus) as a model protein. The transgenic sugarcane model offers the following advantages:
• The yield and activity of the recombinant protein is high
• The recombinant protein is targeted and stored in vacuole. In a mature cane 80-85% of the cells are filled with vacuole. One kilogram of stalk can give 600-650 ml of juice.
• Sugarcane juice has relatively negligible amount of protein (around 0.04%), hence it would be simpler to purify the heterologous proteins expressed in juice.
• Large storage vacuole makes it possible to store even proteins that are phyto- toxic and thus isolating it from other metabolic pathways.
• Sugarcane being vegetatively propagated, biosafety issues with transgenic sugarcane are low.
• Specific morphologically distinct varieties for different proteins are possible.
• As raw sugarcane juice is palatable direct delivery of vaccines or nutricuticles is possible.
For further information please contact
Biotech Consortium India Limited
V Floor, Anuvrat Bhawan
210, Deen Dayal Upadhay Marg
New Delhi-110002
Phone: +91 (011) 23219064-67
